目的：通过建立的高效液相色谱法对吴茱萸及其炮制品中吴茱萸碱、吴茱萸次碱和柠檬苦素3个指标性成分含量进行测定,并进行对比分析。方法：采用高效液相色谱法,色谱柱Agi Lent C18（4.6 mm×250 mm,5μm）;流动相：乙腈-水（55：45）;流速：1 m L·min~（-1）;检测波长：225 nm;柱温：25℃。结果：吴茱萸炮制品与生品相比较,3个指标性成分含量均下降,其中柠檬苦素含量下降较多。结论：吴茱萸炮制后对吴茱萸碱、吴茱萸次碱含量影响不大,对柠檬苦素含量影响较大。
摘要(英文)：
Objective：To develop an HPLC method to determine the content of evodiamine, rutaecarpine and Limonin in crude Evodia and its processed products, and do comparative analysis. Methods： The separation was performed on the AgiLent C18 （4.6mm×250mm, 5 μm） coLumn with acetonitrile-water （55：45） as mobile phase. The flow rate was 1 mL min-1. The wave length of UV detector was set at 225 nm and the column temperature was set at 25 ℃. Results： Compared with crude Evodia, the three index components all decline slightly in pro- cessed products ...
