期刊论文

Effect of Buyang Huanwu Decoction (补阳还五汤)on L-type Calcium Channel of Red Nucleus Neurons in Rats with Spinal Cord Injury

中文

中医药导报

1672-951X

2018

24

20

26-29

10.13862/j.cnki.cn43-1446/r.2018.20.007

湖南省自然基金资助项目（2018JJ2289）； 国家自然科学基金资助项目（81302899）； 湖南省中医药科研计划项目（2013111）； 湖南中医药大学中医诊断学国家重点学科开放基金（2014-37）；

本校为第一机构

目的:探究补阳还五汤对脊髓损伤大鼠红核神经元L-型钙通道(ICa-L)电流及活动变化的影响。方法:将大鼠随机分为假手术组、模型组、补阳还五汤低、中、高剂量组,每组10只。采用红核脊髓束(rubrospinal tract,RST)横断方法,建立脊髓损伤动物模型,分离红核细胞。采用膜片钳技术记录各组ICa-L的变化及离子活动。结果:模型组红核细胞ICa-L电流密度在测试电压-20~+70 mV之间均明显低于假手术组(P

Objective:To study the effects of Buyang Huanwu Decoction (BYHWD)on L-type calcium channel (ICa-L)of Red Nucleus Neurons in rats with Spinal cord injury.Methods:The experiment rats were randomly divided into sham operation group,model group,BYHWD low groups,BYHWD mediate groups and BYHWD high groups,10rats in each group.The ruhrospinal tract was damaged in modal group and BYHWD group and given red nucleus neurons;the change of ICa-L currents were detected with Patch-clamp techniques. Results:Compared with the sham operation group,the model group showed lower ICa-L density of erythrocyte betwee...