期刊论文

中文

中医药导报

1672-951X

2019

03

39-41

10.13862/j.cnki.cn43-1446/r.2019.03.011

国家自然科学基金项目（81473615）； 湖南省自然基金项目（2015JJ2017）；

本校为第一机构

目的:探讨TLR4基因敲除小鼠动脉粥样硬化时Wnt1的变化及护心康的干预作用。方法:SPF级TLR4基因敲除小鼠共70只,随机取12只为正常组,予以普通饲料喂养;其余58只予以高脂饲料造模,喂养13周后从造模动物中随机选取5只,观察胸主动脉,光镜下发现粥样斑块为造模成功。造模成功动物随机分为模型组、护心康组、Wnt抑制剂WIF组、阿托伐他汀组,每组12只,分别予以相应处理。干预8周后处死小鼠取胸主动脉,光镜下观察动脉粥样硬化形成情况;免疫组化法检测斑块中Wnt1蛋白的表达。结果:（1）光镜观察:正常组未见动脉粥样硬化;模型组可见动脉内膜增厚,粥样斑块形成,血管腔减小;各药物干预组斑块病变相对较轻。（...

Objective:To investigate the changes of Wnt1 in TLR4-/-mice of atherosclerosis and the intervention of Huxinkang.Methods:A total of 70 SPF TLR4-/-mice were fed,and 12 mice of them were randomly selected as control group and fed with normal diet.The remaining 58 mice were modeled with high-fat diet.After 13 weeks of feeding,5 mice were randomly selected from them to observe the thoracic aorta,and atheroma plaque was found under light microscopy indicating successful modeling.The successful model animals were randomly divided into model group,HuxinKang group,Wnt inhibitor WIF group and atorvasta...