期刊论文

中文

中医药导报

1672-951X

2020

26

5

35-38

10.13862/j.cnki.cn43-1446/r.2020.05.009

2018JJ2297:湖南省自然科学基金面上项目 19A370:湖南省教育厅科学研究项目 :湖南中医药大学校级科研基金项目 2018XJJ24:精准科技扶贫项目 2018ZXYJH25:湖南中医药大学中西医结合一流学科开放基金项目 2019-68:湖南中医药大学大学生创新创业训练计划项目

本校为第一机构

目的:观察壮骨止痛方对去势雌鼠转化生长因子β1(Transforming growth factor-β,TGF-β1)mRNA及Ⅰ型胶原纤维mRNA表达的影响,并探究其治疗骨质疏松的具体机制。方法:将75只健康雌性SD大鼠随机分为空白组、假手术组、模型组、壮骨止痛方组、无水乙醇提取A部位组,每组15只。除空白组、假手术组外,其余各组建立绝经后骨质疏松病理模型,假手术组去除卵巢周围相应体积脂肪。造模后5 d开始给药,13周后取股骨,原位杂交法检测分析骨组织中Ⅰ型胶原纤维mRNA和TGF-β1 mRNA的表达。结果:模型组TGF-β1 mRNA和Ⅰ型胶原mRNA表达明显低于空白组(P

Objective:To observe the effect of alcohol extract A of Zhuanggu Zhitong Prescription(ZGZFP)on the expression of TGF-β1 mRNA and typeⅠcollagen fiber mRNA in defoliated female mice,and to explore the specific mechanism of its treatment of osteoporosis.Methods:The 75 healthy female SD rats were randomly divided into blank group,sham operation group,model group,ZGZTP group and anhydrous ethanol extraction group,with 15 rats in each group.In addition to the blank group and the sham group,pathological models of postmenopausal osteoporosis were est...