期刊论文

Effect of Chaishao Liujunzi Decoction(柴芍六君子汤)on 5-HT in Hippocampus,Hypothalamus,Stomach and Serum of Functional Dyspepsia Model Rats

中文

中医药导报

1672-951X

2020

26

6

1-5

10.13862/j.cnki.cn43-1446/r.2020.06.001

81904176:国家自然科学基金 No.2019JJ50463:湖南省自然科学基金青年项目 2018SK51201:科技创新项目 CX20190593:湖南省研究生科研创新项目 2018;2020:湖南中医药大学中医学国内一流建设学科资助项目

本校为第一机构

目的:观察柴芍六君子汤对功能性消化不良(functional dyspepsia,FD)大鼠海马、下丘脑、胃组织及血清中五羟色胺表达的影响,探讨柴芍六君子汤治疗功能性消化不良的作用机制。方法:将60只SD大鼠随机分成空白对照组、模型组、多潘立酮组和柴芍六君子汤组,每组15只,除空白对照组外,其余3组用复合病因造模法造成FD模型,连续21d。灌胃14d后腹主动脉取血,离心制备血清样本,取胃、海马及下丘脑组织。测定胃排空率,采用ELISA法检测血清五羟色胺(5-HT)的含量,免疫组织化学法检测海马、下丘脑及胃组织中5-HT的表达。结果:模型组大鼠胃排空率显著低于空白对照组(P

Objective:To observe the effect of Chaishao Liujunzi decoction on 5-HT in hippocampus,hypothalamus,stomach and serum of functional dyspepsia model rats,and to explore the effect and mechanism of Chaishao Liujunzi decoction on functional dyspepsia.Methods:Totally 60 SD rats were randomly divided into blank group,model group,domperidone group and Chaishao Liujunzi group,with 15 rats in each group.Except the blank group,the other three groups were given compound etiology modeling method to cause functional dyspepsia model,21 days consecutively.Blood was taken from the abdominal aorta 14 days afte...