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Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties

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成果类型:
期刊论文
作者:
Long, Yu Qing;Liu, Xia;Zeng, Juan;Li, Can;Liu, Xiang Dan*;...
通讯作者:
Liu, Xiang Dan;Zhou, RB;Liu, XD
作者机构:
[Zeng, Juan; Liu, Xiang Dan; Long, Yu Qing; Zhou, Ri Bao; Zhou, RB; Li, Can; Liu, Xia] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.
[Zeng, Juan; Liu, Xiang Dan; Long, Yu Qing; Zhou, Ri Bao; Zhou, RB; Li, Can; Liu, Xia] Key Lab Germplasm Resources & Standardized Planti, Changsha 410208, Peoples R China.
[Liu, Xiang Dan] Hunan Tradit Chinese Med Piece Standardizat & Fun, Changsha 410208, Peoples R China.
通讯机构:
[Liu, XD ; Liu, XD; Zhou, RB ] H
Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.
Key Lab Germplasm Resources & Standardized Planti, Changsha 410208, Peoples R China.
Hunan Tradit Chinese Med Piece Standardizat & Fun, Changsha 410208, Peoples R China.
语种:
英文
关键词:
MADS domain protein;plant protein;amino acid sequence;classification;flower;gene expression regulation;genetics;growth, development and aging;Lonicera;metabolism;molecular cloning;nucleotide sequence;phylogeny;sequence homology;Amino Acid Sequence;Base Sequence;Cloning, Molecular;Flowers;Gene Expression Regulation, Plant;Lonicera;MADS Domain Proteins;Phylogeny;Plant Proteins;Sequence Homology
期刊:
JOURNAL OF GENETICS
ISSN:
0022-1333
年:
2021
卷:
100
期:
1
页码:
1-10
基金类别:
This work was supported by ‘the Chinese National Natural Science Foundation’ (Grant Nos. 81203007 and 81673546), ‘The Natural Science Foundation of China Hunan Province’ (Grant No. 2017JJ3237), ‘First-class Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine’ ([2018] No. 3), ‘A Project Supported by Scientific Research Fund of Hunan Provincial Education Department’ (Grant No. 19A367), ‘Scientific Research Project Fund of Hunan Agriculture and Countryside Department’ ([2019] no. 105). We thank International Science Editing for editing this manuscript.
机构署名:
本校为第一且通讯机构
院系归属:
药学院
摘要:
AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The full-length cDNAs of AGL19 in conventional Lonicera macranthoides (Lm-AGL19) and the mutant ‘Xianglei’ cultivar (Lm-XL-AGL19) were obtained using rapid amplification of cDNA ends and the expression vectors for Lm-XL-AGL19 were constructed to investigate the roles of AGL19 in the ‘Xianglei’ cultivar. Lm-AGL19 (GenBank: MK419948) and Lm-XL-AGL19 (GenBank: MK419948) were isolated from the conventional variety and ‘Xiangl...

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