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Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties

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成果类型:
期刊论文
作者:
Long, Yu Qing;Liu, Xia;Zeng, Juan;Li, Can;Liu, Xiang Dan*;...
通讯作者:
Liu, Xiang Dan;Zhou, Ri Bao
作者机构:
[Zeng, Juan; Liu, Xiang Dan; Zhou, RB; Long, Yu Qing; Zhou, Ri Bao; Li, Can; Liu, Xia] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.
[Zeng, Juan; Liu, Xiang Dan; Zhou, RB; Long, Yu Qing; Zhou, Ri Bao; Li, Can; Liu, Xia] Key Lab Germplasm Resources & Standardized Planti, Changsha 410208, Peoples R China.
[Liu, Xiang Dan] Hunan Tradit Chinese Med Piece Standardizat & Fun, Changsha 410208, Peoples R China.
通讯机构:
[Liu, XD; Zhou, RB; Liu, Xiang Dan] H
[Liu, XD; Zhou, RB] K
Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.
Key Lab Germplasm Resources & Standardized Planti, Changsha 410208, Peoples R China.
Hunan Tradit Chinese Med Piece Standardizat & Fun, Changsha 410208, Peoples R China.
语种:
英文
关键词:
AGL19 gene;bioinformatics;gene cloning;plant expression vector;quantitative real-time polymerase chain reaction;Lonicera macranthoides Hand. -Mazz.
期刊:
JOURNAL OF GENETICS
ISSN:
0022-1333
年:
2021
卷:
100
期:
1
页码:
1-10
基金类别:
Chinese National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81203007, 81673546]; Natural Science Foundation of China Hunan Province [2017JJ3237]; 'First-class Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine' ([2018]) [3]; Scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [19A367]; 'Scientific Research Project Fund of Hunan Agriculture and Countryside Department' ([2019]) [105]
机构署名:
本校为通讯机构
院系归属:
药学院
摘要:
AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The full-length cDNAs of AGL19 in conventional Lonicera macranthoides (Lm-AGL19) and the mutant ‘Xianglei’ cultivar (Lm-XL-AGL19) were obtained using rapid amplification of cDNA ends and the expression vectors for Lm-XL-AGL19 were constructed to investigate the roles of AGL19 in the ‘Xianglei’ cultivar. Lm-AGL19 (GenBank: MK419948) and Lm-XL-AGL19 (GenBank: MK419948) were isolated from the conventional variety and ‘Xiangl...

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