期刊论文

中文

中南林业科技大学学报

1673-923X

2010

30

1

48-53

10.14067/j.cnki.1673-923x.2010.01.011

2006BAI06A11-06:国家科技支撑计划 2008089:湖南省中医药局项目

本校为其他机构

以百合新鲜鳞叶为材料,利用RAPD分子标记对来自我国5省共16份百合样品进行了分析.结果表明:采用改良CTAB法提取百合鳞叶中DNA条带清晰较,且从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%.聚类分析表明,16个百合材料在阈值为0.9407时分为2个RAPD群,即分别属于百合科的百合属和大百合属;阈值为0.5790时,百合属分为3个种,即分别为百合、卷丹和细叶百合.这些均可为进一步研究百合的分子生物学特性打下基础,为鉴定中药材百合品种品系的新方法提供充分的实验依据.

In this study,16 samples of Lily collected from different ecological sources were analyzed by RAPD using its leaf scales.The bands of DNA extracted by ameliorated CTAB.Thirty-five primers were screened from 120 random primers,and a total 769 DNA bands were amplified,628（81.7%） of which were polymorphic.Cluster analysis results show that the 16 Lily materials were divided into two RAPD groups at 0.940 7.There are two different genera plants： Lilium and Cardiocrinum;Lilium was divided into three different types at 0.579 0,Lilium brownii var.vi...