会议论文

中文

2016

268-275

中国中西医结合学会诊断专业委员会第十次全国学术会议

中国中西医结合学会诊断专业委员会第十次全国学术会议论文集

2016-07-01

河北秦皇岛

中国中西医结合学会

国家自然科学基金资助项目(No.81273665);湖南中医药大学中医诊断国家重点学科开放基金项目(No.201401)

本校为第一机构

目的:筛选胃癌患者与慢性胃炎患者及健康志愿者的唾液蛋白质组差异表达谱,并建立胃癌患者与慢性胃炎唾液无创伤分子诊断(判别)模型。方法:采集57例胃癌患者(术前且无放化疗)和28例慢性胃炎患者的唾液标本,用WCX(弱阳离子交换型)纳米磁珠联合基质辅助激光解析离子化飞行时间质谱(matrix assisted laser desorption ionization time-of-flight mass

　　Objective: To screen differential protein expression profiles of saliva proteome between gastric carcinoma groups, normal control group, and chronic gastritis, and to establish a model for differentiating these groups.Methods: Saliva samples from 57 patients with gastric carcinoma, 28 patients with gastritis, and 45 healthy volunteers, were purified by weak cationic-exchange magnetic beads (MB-WCX) and then analyzed by matrix-assisted laser desorptionionization time-of-flightmass spectrometry (MALDI-TOF-MS),Subsequently, we compared the sal...