期刊论文

He, F.-Y.

中文

药学学报

0513-4870

2008

43

2

195-201

10.3321/j.issn:0513-4870.2008.02.016

国家自然科学基金资助项目（30472199,30572378） 中国博士后基金资助项目（20070410997）

本校为第一且通讯机构

本文运用了统计矩原理阐明并建立中药复方多成分指纹图谱定性定量分析法:总量统计矩方法.包括4个参数: ①总零阶矩AUCT; ②总响应率AUCPWT; ③总量一阶矩MCRTT,亦总量中心矩或总量平均保留时间,用T表示; ④总体二阶矩VCRTT,亦为平均保留时间方差,用2T表示.AUCT能用于中药复方指纹图谱定量分析,AUCPWT、MCRTT、VCRTT能用于中药复方指纹图谱定性分析.以此法研究不同产地大黄醇浸膏成分HPLC指纹图谱,得总量统计矩参数:AUCT为3.273×108 μV·s;AUCPWT为2.286×106 μV·s·mg-1;MCRTT为33.50 min; VCRTT为484.4 min2;浓度CT为143.2 mg·mL-1.本法具有加合运算的特征,能消除溶剂干扰,获得纯品的总量...

A new qualitative and quantitative analytical method of the chromatographic fingerprints: the Total Quantum Statistical Moment (TQSM) has been eluciated and established according to statistical moment principle. The study was carried out with model drugs as the alcohol extracted liquid for Radix et Rhizoma Rhei (AELRR) by HPLC under the chromatographic conditions that the column was C18, 4.6 mm x 250 mm, 5 microm; the detection of wavelengths was set at 254 nm; a solution of acetonitrile: 1% acetic acid water (from 0:100 to 100:0) was carried with gradient elution as the mobile phase; the rati...