期刊论文

Effects of Buyang Huanwu decoction on protein kinase B1 and c-Jun amino terminal kinase 112 in rats after focal cerebral ischemia

中文

中国中西医结合急救杂志

1008-9691

2013

5

275-278

10.3969/j.issn.1008-9691.2013.05.005

湖南省教育厅优秀青年项目（108080）

本校为第一机构

目的探讨补阳还五汤对脑缺血后大鼠蛋白激酶B1（AKT1）和c-Jun氨基末端激酶1/2（JNK1/2）的影响。方法将48只SD大鼠按随机数字表法分为正常对照组、假手术组、模型组、补阳还五汤组，每组12只。采用大脑中动脉闭塞法（MCAO）建立右侧局灶性脑缺血大鼠模型。补阳还五汤组大鼠于术后2h起灌服补阳还五汤（主要由生黄芪120g、当归6g、赤芍4.5g、川芎3g、西红花3g、桃仁3g、广地龙3g组成）14.2 g/kg，每日1次，连续7 d。其他各组动物均给予等体积生理盐水。于第7日处死大鼠，取脑组织，采用逆转录-聚合酶链反应（RT-PCR）测定AKT1 mRNA表达，采用免疫组化法检测JNK1/2蛋白表达。结果与正常对照组和假...

Objective To explore the effect of Buyang Huanwu decoction(BYHWD)on protein kinase B1 (AKT1)and c-Jun amino terminal kinase 1/2(JNK1/2)in rats after focal cerebral ischemia. Methods According to the random number table method,48 Sprague-Dawley(SD)rats were randomly allocated to four groups:normal control group,sham-operated group,model group,traditional BYHWD group(each n=12). The rat model of right focal cerebral ischemia was established by the method of middle cerebral artery occlusion(MCAO). The rats in BYHWD group were ingested with the dec...