期刊论文

Regulation effect of Wenyang Zhenshuai Granule on SIRS/CARS balancein inflammatory reaction of rats with sepsis

中文

中国中西医结合急救杂志

1008-9691

2019

26

6

659-662

10.3969/j.issn.1008-9691.2019.06.005

国家自然科学基金(81573956,81503445) 湖南省中医药科研计划项目(201560) 湖南省自然科学基金(2016JJ2095)。

本校为第一机构

目的探讨温阳振衰颗粒对脓毒症大鼠炎症反应的调节作用。方法将56只SD大鼠按随机数字表法分为假手术组(n=8)、模型组(n=24)、中药治疗组(n=24)。采用腹腔注射脂多糖(LPS)5 mg/kg的方法制备脓毒症大鼠模型;假手术组给予等量生理盐水。中药治疗组于制模后即刻灌胃温阳振衰颗粒原药(250 g/L)2 g·kg~(-1)·d~(-1);模型组灌胃等量生理盐水;此后存活动物每12 h给药(或生理盐水);假手术组不予处理。各组分别于制模后24、48、72 h取8只大鼠,经眼眶采血后处死,采用酶联免疫吸附试验(ELISA)测定血清中肿瘤坏死因子- α(TNF-α)、白细胞介素(IL-6、IL-10)水平;采用聚合酶链反应(PCR)检测肠组织中Toll样受体...

Objective To observed the regulation effect of Wenyang Zhenshuai Granule on inflammatory reaction of rats with sepsis. Methods Fifty-six Sprague-Dawley (SD) rats were divided into sham operation group (Sham group, n = 8), model group (n = 24), and Chinese medicine treatment group (n = 24) according to random number table method. Rats modelling with sepsis were developed by intraperitoneal injection of lipopolysaccharide (LPS, 5 mg/kg). Rats in Sham group were given the same amount of normal saline by intraperitoneal injection. After modeling, r...