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Genome-wide CRISPR-Cas9 viability screen reveals genes involved in TNF-α-induced apoptosis of human umbilical vein endothelial cells

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成果类型:
期刊论文
作者:
Cai, Meng;Li, Sitao;Shuai, Yunfei;Li, Jie;Tan, Jieqiong;...
通讯作者:
Zeng, QY;Tan, JQ
作者机构:
[Zeng, QY; Zeng, Qiyi; Cai, Meng] Southern Med Univ, Zhujiang Hosp, Dept Pediat, Guangzhou 510280, Guangdong, Peoples R China.
[Li, Sitao] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Pediat, Guangzhou, Guangdong, Peoples R China.
[Shuai, Yunfei] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Pediat, Changsha, Hunan, Peoples R China.
[Li, Jie; Tan, Jieqiong; Tan, JQ] Cent S Univ, Ctr Med Genet, Changsha 410078, Hunan, Peoples R China.
[Li, Jie; Tan, Jieqiong; Tan, JQ] Cent S Univ, Sch Life Sci, Changsha 410078, Hunan, Peoples R China.
通讯机构:
[Zeng, QY ] S
[Tan, JQ ] C
Southern Med Univ, Zhujiang Hosp, Dept Pediat, Guangzhou 510280, Guangdong, Peoples R China.
Cent S Univ, Ctr Med Genet, Changsha 410078, Hunan, Peoples R China.
Cent S Univ, Sch Life Sci, Changsha 410078, Hunan, Peoples R China.
语种:
英文
关键词:
aven protein;mitogen activated protein kinase kinase kinase;mitogen activated protein kinase kinase kinase 1;mitogen activated protein kinase kinase kinase 7;ndn protein;nlr family member x1;prkar1b protein;protein;protein BAD;protein bcl 2;protein Bid;protein p53;tumor necrosis factor;tumor necrosis factor receptor;tumor necrosis factor receptor superfamily member 1A;unclassified drug;apoptosis regulatory protein;BAD protein, human;BCL2 protein, human;mitochondrial protein;NLRX1 protein, human;protein BAD;protein bcl 2;TNFRSF1A protein, human;tumor necrosis factor;tumor necrosis factor receptor 1;apoptosis;Article;cell viability;cellular distribution;confocal microscopy;controlled study;CRISPR-CAS9 system;genome-wide association study;human;human cell;HUVEC cell line;methyl thiazolyl tetrazolium assay;priority journal;Sanger sequencing;vascular endothelial cell;Western blotting;apoptosis;cell culture;clustered regularly interspaced short palindromic repeat;CRISPR Cas system;drug effect;genetics;metabolism;mitochondrion;pathology;signal transduction;umbilical vein endothelial cell;Apoptosis;Apoptosis Regulatory Proteins;bcl-Associated Death Protein;Cells, Cultured;Clustered Regularly Interspaced Short Palindromic Repeats;CRISPR-Associated Protein 9;CRISPR-Cas Systems;Human Umbilical Vein Endothelial Cells;Humans;Mitochondria;Mitochondrial Proteins;Proto-Oncogene Proteins c-bcl-2;Receptors, Tumor Necrosis Factor, Type I;Signal Transduction;Tumor Necrosis Factor-alpha
期刊:
JOURNAL OF CELLULAR PHYSIOLOGY
ISSN:
0021-9541
年:
2019
卷:
234
期:
6
页码:
9184-9193
机构署名:
本校为其他机构
院系归属:
第一中医临床学院
摘要:
Tumor necrosis factor α (TNF-α), a pivotal cytokine in sepsis, protects the host against pathogens by promoting an inflammatory response while simultaneously inducing apoptosis of the vascular endothelium. Unfortunately, inhibitors targeting certain components of the TNF-α signaling pathway to reduce cellular apoptosis have failed to translate into clinical applications, partly due to the adverse effects of excessive immunosuppression. In an attempt to discover potential targets in the TNF-α signaling pathway to modulate moderate inflammati...

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