期刊论文

中文

应用与环境生物学报

1006-687X

2016

22

3

437-441

10.3724/SP.J.1145.2015.08018

(81302952):国家自然科学基金项目 (81302952):Supported by the National Natural Science Foundation of China

本校为第一机构

为了解脾虚便秘小鼠肠道细菌的特性,采用灌胃番泻叶水煎液7 d,控制饮食、饥饱失常8 d建立小鼠脾虚便秘模型,造模结束后提取肠道微生物总DNA,进行16S rDNA V4区基因组测序分析.结果显示,从OTU总数来看,模型组有896种,少于正常组的982种,二者有807种相同;从PCA分析结果来看,正常组分布相对集中,模型组彼此离散,且与正常组的距离较大;从生物多样性指数来看,模型组的Chao指数、ACE指数、Shannon指数均显著低于正常组(P< 0.05),模型组的Simpson指数值则显著高于正常组(P< 0.05);从细菌相对丰度来看,模型组的Clostridia、 Coriobacteriales、Bacteroidales、Clostridials、Coriobacteriia、Deltaprote...

This research aimed to study the effects of building spleen-deficient constipation model on the intestinal bacterial diversity in mice. For construction of models of spleen-deficiency constipation, 6 mice were made hunger satiety disordered by being fed with senna water decoction for 7 days followed by diet restriction for 8 days. The intestinal microbes total DNA was extracted for genome sequencing analysis of 16S rDNA V4 area. The model group had 896 different species of OTU, less than the 982 species of the normal group, with 807 of them bei...