期刊论文

Establishment of a mouse model of Ganqichengpi diarrhea and the efficacy of Tongxieyaofang prescription

HUI, H.;TAN, Z.

中文

应用与环境生物学报

1006-687X

2020

26

4

1023-1027

10.19675/j.cnki.1006-687x.2019.09026

收稿日期 Received: 2019-09-11 接受日期 Accepted: 2019-11-07 国家自然科学基金项目（81874460，81703985）和2019年湖南中医药大学校级研究生篇培养质量工程项目（2019CX35）资助 Supported by the National Natural Science Foundation of China (81874460, 81703985) and the 2019 Hunan University of Traditional Chinese Medicine University-Level Postgraduate Part Training Quality Engineering Project (2019CX35) 通讯作者 Corresponding authors (E-mail: huihuaying2003@126.com; tanzhjin@sohu.com)

本校为第一且通讯机构

为建立小鼠肝气乘脾泄泻的造模方法,并通过痛泻要方对其疗效进行验证.采用番泻叶加束缚夹尾法制备肝气乘脾泄泻小鼠模型,运用痛泻要方进行治疗,以主观和客观指标进行综合评价.结果显示,番泻叶-束缚夹尾使模型小鼠出现情绪亢奋,腹泻程度严重,进食量减少,体重增长幅度下降,毛发变暗、枯黄,同时甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、5-羟色胺(5-HT)含量升高(P > 0.05),D-木糖含量下降(P < 0.05),符合脾虚证诊断标准,同时出现肠推进率增高(P < 0.05)、胃残留率降低(P > 0.05)等小肠功能亢进的表现.经痛泻要方干预后小鼠一般行为接近正常组,腹泻症状明显消失,总胆固醇(TC)、高密度脂蛋白胆固醇(...

This study aimed to establish a Ganqichengpi diarrhea mouse model and to examine the efficacy of Tongxieyaofang. A mouse model of Ganqichengpi diarrhea was prepared via the senna leaf plus restraint tail method, and treatment was conducted by Tongxieyaofang prescription. The results showed that the senna leafbinding tail made the model mice emotionally excited, the degree of diarrhea was serious, the food intake and weight gain decreased, and the hair became dark and yellow. The triglyceride (TG), low density lipoprotein cholesterol (LDL-C), an...