期刊论文

中文

农药学学报

1008-7303

2013

15

3

299-304

10.3969/j.issn.1008-7303.2013.03.10

科技部国际科技合作计划

本校为第一机构

采用酸提碱沉的方法,从博落回中提取并制备了苯并啡啶类生物碱(QBAs)--血根碱(sanguinarine)和白屈菜红碱(chelerythrine)的硫酸氢盐;采用高效液相色谱法测定了2种生物碱的含量;初步测定了其硫酸氢盐对几种常见病原菌的抗菌活性,并对其急性毒性进行了研究。结果表明:所制备的QBAs硫酸氢盐中血根碱和白屈菜红碱的质量分数均在60%以上。生物测定结果表明,该生物碱的硫酸氢盐对葡萄叶斑病菌Cercospora viticola、葡萄黑豆病菌Elsinoe ampelina、葡萄炭疽病菌Colletotrichum gloeosporioides、葡萄褐斑病菌Cercospora viticola、稻瘟病菌Pvricularia oryzae、小麦赤霉病菌Gibberella zeae和辣椒疫霉...

To develop low toxicity and environment-friendly botanical fungicide, the quaternary benzophenanthridine alkaloids （QBAs） was prepared with the method of acid extraction and alkali sink; the contents of sanguinarine and chelerythrine alkaloids in the QBAs were determined by high performance liquid chromatography （HPLC）. Antibacterial activity of QBAs bisulfate was tested by mycelium growth rate method and pot growing method. The acute toxicity of QBAs bisulfate was tested. The results showed that, the mass fraction of sanguinarine and chele...