期刊论文

中文

现代生物医学进展

1673-6273

2010

10

10

1825-1827+1835

10.13241/j.cnki.pmb.2010.10.011

湖南省教育厅优秀青年项目（09B075）；

本校为其他机构

目的:探讨三七总皂苷对脊髓损伤后的保护作用以及对GFAP表达变化的影响.方法:健康成年雌性SD大鼠63只,随机分为正常组,溶媒对照组,三七总皂苷组,大鼠脊髓T10右侧半横断损伤后15min,腹腔注射三七总皂苷,剂量为20mg.kg-1,以后每天给药一次,溶媒对照组注射等量生理盐水.术后1d,3d,7d,14d,21d,28d进行BBB评分行为学检测;动物存活1d、3d、7d、14d、 28d,运用免疫组织化学方法检测脊髓损伤远侧端GFAP表达的变化.结果:BBB评分显示,三七总皂苷能明显促进脊髓损伤后运动功能的恢复,其中损伤后7d和14d的评分明显高于溶媒对照组.免疫组化结果显示脊髓T10右侧半横断损伤后.脊髓远侧段 GFAP的表达损伤侧均强于...

Objective：To investigate the protective effect of TSPN on spinal cord injury and the influence on GFAP expression.Methods：63 adult female SD rats were randomly divided into normal group,solvent control group and TSPN group.TSPN was injected intraperitoneally at the dose of 20mg·kg^-1 after T10 spinal cord hemisection,once a day.The vehicle control group was administrated with equivalent saline.BBB score was assessed at 1d,3d,7d,14d,21d,28d after spinal cord hemisection.GFAP expression changes in distal spinal cord were detected by immunohist...