期刊论文

Deng, C.Q.

中文

中国中西医结合杂志

1003-5370

2002

22

3

193-195,210

10.3321/j.issn:1003-5370.2002.03.011

国家自然科学基金资助项目 （No .39770 893）；

本校为第一机构

目的：比较补阳还五汤及其有效部位组方对脑缺血后热休克蛋白70（HSP70）表达的影响。方法：采用沙土鼠双侧颈总动脉夹闭脑缺血模型，分别腹腔注射补阳还五汤及其有效部位组方，于缺血15min再灌注48h后，以Northern blot检测脑组织HSP70mRNA的表达，以Western blot检测其蛋白表达。结果：沙土鼠脑缺血再灌注后，脑组织HSP70mRNA和蛋白表达显著增加，补阳还五汤及其有效部位组方可使脑缺血再灌注后HSP70mRNA表达减少，补阳还五汤的抑制作用强于有效部位组方；但两者对脑缺血再灌注后HSP70蛋白表达的增加均无明显影响。结论：补阳还五汤及其有效部位组方可能通过抗缺血作用而抑制了缺血后HSP70mRN...

OBJECTIVE: To compare the effects of Buyang Huanwu Decoction (BYHWD) and its active regions combination on brain heat shock protein 70 (HSP70) expression. METHODS: Cerebral ischemia model gerbils produced by ligating bilateral common carotid artery were injected intraperitoneally by BYHWD and its active regions combination separately. HSP70 mRNA expression was determined by Northern blot test and protein expression determined by Western blot test 48 hrs after ischemia for 15 min and reperfusion. RESULTS: The level of HSP70 mRNA and protein expression in ischemic/reperfusion brain tissue increa...