期刊论文

Wang, R.G.

中文

中国中西医结合杂志

1003-5370

2005

25

6

516-519

10.3321/j.issn:1003-5370.2005.06.010

(C30000225):国家自然科学基金 (2000-J-T-17):国家重点基础研究发展计划(973计划) (01B014):湖南省教育厅青年基金

本校为第一机构

目的研究黄芪丹参复方提取物提高胎盘供血的分子机制,为临床有效防治胎盘供血不足所致妊娠并发症提供思路.方法提取分离中药黄芪、丹参复方成分,运用一氧化氮合酶(NOS)阻滞剂L-精氨酸甲酯(L-NAME)建立一氧化氮合成阻滞大鼠模型,核酸原位杂交技术检测妊娠12日胎盘滋养细胞、始基微血管内皮细胞血管内皮生长因子(VEGF)mRNA表达变化.结果一氧化氮合成阻滞模型VEGF mRNA表达较空白组、中药治疗组及硝酸甘油治疗对照组均明显降低(P＜0.05,P＜0.01).结论黄芪丹参复方成分可能对妊娠早期胎盘滋养细胞及血管内皮细胞VEGF mRNA表达具有促进作用,从而有利于胎盘血管网络形成及滋养细胞侵入及母胎循环构建,...

OBJECTIVE: To investigate the molecular mechanisms of effective ingredients of Astragalus-Salvia compound (ASC) in increasing placental blood supply, to provide thought for establishing an effective prevention and treatment of insufficient placental blood supply caused complications of pregnancy. METHODS: The effective ingredients of ASC was isolated and abstracted and the rat model of inhibited nitric oxide synthesis was established by using L-Arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor. The mRNA expression of vascular endothelial growth factor (VEGF) in placental tropho...