期刊论文

中文

中国中西医结合杂志

1003-5370

2017

37

9

1105-1108

10.7661/j.cjim.20170713.180

湖南省中医药科研计划重点项目（No.201612） 湖南省教育厅重点课题（No.20161616）

本校为第一机构

目的观察壮骨止痛方对体外培养成骨细胞Wnt/β-catenin信号通路的影响。方法取新生SD大鼠颅骨进行成骨细胞原代培养,传代后随机分为4组:假手术组、模型组、对照组、中药组。培养3代后进行鉴定,加入相应含药血清干预48 h。测定各组成骨细胞Wnt3a、β-catenin、LRP5、GSK3β mRNA及蛋白的表达情况。结果与假手术组比较,模型组β-catenin、LRP5蛋白及mRNA降低(P

Objective To observe the effect of Zhuanggu Zhitong Recipe (ZGZTR) on Wnt/β-catenin signaling pathway of osteoblast cultured in vitro . Methods Osteoblasts from neonatal SD rats were cultured in primary culture then divided into 4 groups randomly, sham-operated group, model group, control group and Chinese medicine (CM) group. Osteoblasts were identified after 3 generations, then intervened by drug containing serum for 48 h. The mRNA and protein expression of Wnt3a, β-catenin,LRP5, GSK3β were detected. Results Compared with sham-operated gro...