期刊论文

中文

中国中西医结合杂志

1003-5370

2018

38

5

574-578

10.7661/j.cjim.20180124.164

国家自然科学基金青年基金资助项目（No.81303051） 湖南省中医药科研计划项目（No.201471）

本校为第一机构

目的观察针刺联合亚低温疗法对脑缺血再灌注损伤大鼠脑组织miRNA功能表达的影响。方法将60只SD大鼠随机分为空白组、假手术组、模型组、针刺组、亚低温组、针刺联合亚低温组(联合组),每组10只。采用Zea Longa线拴法复制改良大脑中动脉缺血模型,造模成功后3 h进行干预。假手术组、模型组只捆绑;针刺组针刺人中、百会、大椎穴,每次留针30 min,12 h/次,共治疗7次;亚低温组置于冰盒内,肛温(33±1)℃、鼓膜温度(31 ±1)℃保持72 h;联合组接受针刺及亚低温治疗72 h。治疗前后进行大鼠神经功能缺损评分,测量治疗后大脑梗死面积比,采用基因微阵列技术筛选差异表达miRNA。结果与空白组及假手术组比较,模型...

Objective To investigate the effect of acupuncture combined with mild hypothermia therapy on functional expression of miRNA in cerebral ischemia reperfusion injury (CIRI) rats. Methods Totally 60 healthy Sprague - Dawley (SD) rats were randomly divided into 6 groups:the blank group,the sham group,the model group,the acupuncture group,the mild hypothermia group,and the acupuncture combined with mild hypothermia group (combined group),10 in each group. Focal cerebral ischemia model was prepared with modified Zea Longas suture method. Three hours ...