期刊论文

Effect of Buddlejae Officinalis Eyedrops on the Apoptosis-related Factors Caspase-3,Caspase-8 in Lacrimal Glands of Experimental Dry Eye Rabbit Model

中文

中国中西医结合杂志

1003-5370

2019

39

9

1072-1077

10.7661/j.cjim.20190809.246

国家自然科学基金面上资助项目(No.30772824,No.81574031) 湖南省***卫生人才“225”工程培养项目资助(No.湘卫人发[2013]13号) 湖南省科技厅科研基金资助项目(No.2015SK2016-6) 湖南省发展改革委科研基金资助项目(湘发改投资[2014]658号) 长沙市科技计划重大专项(No.K1501014-31) 湖南中医药大学研究生创新项目(No.2016CX19) 国家中医药管理局中医眼科学重点学科建设项目 中医药防治眼耳鼻喉疾病湖南省重点实验室建设项目.

本校为第一机构

目的通过建立去势兔干眼模型,观察密蒙花滴眼液对其泪腺细胞凋亡相关因子Caspase-3、 Caspase-8的干预作用。方法将35只新西兰白兔随机分为正常组(A组) 、模型组(B组) 、0.3、0.5、 1.0 mg/mL、1.5、3.0 mg/mL密蒙花滴眼液(C、D、E、F、G组) 。A、B组不予处理,C、D、E、F、G组分别予以相应密蒙花滴眼液局部滴眼,3次/日。疗程为4周。造模前、用药前及用药后检测各组基础泪液分泌试验(Schirmer's test I,SIT)水平,免疫组化检测各组泪腺组织中Caspase-3、Caspase-8的表达情况,Real-time PCR法检测各组泪腺组织中Caspase-3、Caspase-8 mRNA的相对表达情况。结果与A比较,用药前及用药后B组SIT值降低(...

Objective To investigate the effects of Buddlejae officinalis eyedrops on the apoptosis-related factors Caspase-3 and Caspase-8 in lacrimal glands of experimental dry eye rabbit model. Methods A total of thirtyfive male rabbit were divided into seven study groups,consisting of the control group (group A) and the treatment group(group B,without any treatment),the dry eye rabbit model group treated with different concentration Buddlejae officinalis eyedrops(0.3,0.5,1,1.5 mg/mL and 3 mg/mL) as group C,D,E,F,3 times a day. The course of treatment was 4 weeks. Schirmer's test I was detected before ...