期刊论文

Effects of Naotai Formula on Iron Overload and Peroxidation Injury in Cortical Neurons of Rats Induced by Hemoglobin

中文

中国中西医结合杂志

1003-5370

2020

40

6

708-714

10.7661/j.cjim.20200113.001

No.81774174:国家自然科学基金 No.81774033:国家自然科学基金 No.201969:湖南省中医药科研项目 No.2018ZXYJH15:湖南中医药大学中西医结合一流学科开放基金项目

本校为第一机构

目的探讨脑泰方对体外血红蛋白( HGB)诱导的大鼠皮质神经元铁超载及过氧化损伤的多靶点干预作用及其机制。方法原代培养新生SD大鼠皮质神经元,采用HGB诱导神经元模拟建立脑出血后神经元铁超载及氧化应激损伤细胞模型;实验设正常组、模型组、空白血清对照组、铁离子螯合剂甲磺酸去铁胺( DFX)组、抗氧化剂N-乙酰半胱氨酸( NAC)组和脑泰方含药血清( NTF)组。采用CCK8法检测各组细胞活力;采用Calcein-AM染色法检测各组细胞内铁含量;采用ELISA法检测各组谷胱甘肽( GSH)含量、谷胱甘肽过氧化物酶4( GPX-4)活性及各组上清液中脂质活性氧( lipid ROS)含量;采用高内涵细胞成像分析技术( HCA)检测各组神经...

Objective To explore the multi-target intervention effect and mechanism of Naotai Formula ( NTF) on hemoglobin( HGB) -induced iron overload and peroxidative damage in rat cortical neuronsin vitro . Methods Cortical neurons of newborn SD rats were cultured,and HGB-induced neuron simulation was used to establish a neuronal iron overload and oxidative stress injury cell model after cerebral hemorrhage.The rats were divided into normal group,model group,blank serum control group,Deferoxamine( DFX) group,N-acetylcysteine ( NAC) group and NTF serum containing group. Cell viability was measured by CC...