期刊论文

中文

中药药理与临床

1001-859X

2017

33

2

41-44

10.13412/j.cnki.zyyl.2017.02.012

湖南省自然科学基金（2015JJ6080） 湖南省教育厅重点项目（15A139） 湖南中医药大学青年教师科研基金课题（22）

本校为第一机构

目的:从miRNA角度探讨黄芩苷对乳腺癌侵袭与转移的影响及可能的机制。方法:黄芩苷作用于乳腺癌MCF-7细胞,运用免疫组化检测TGF-β和VEGF表达的变化,筛选最佳药物浓度;用miRNA芯片筛选受黄芩苷调控的差异miRNA,qRT-PCR对芯片结果中上调的miRNA进行验证,Western-blot检测Bcl-2、Caspase-9、Caspase-3、p-p38、p53的表达,MTT法检测细胞生存率,Transwell实验检测细胞侵袭与转移,流式细胞术检测细胞凋亡。结果:用25μmol /L、50μmol /L、100μmol /L、200μmol /L的黄芩苷作用于乳腺癌细胞,免疫组化显示TGFβ、VEGF的表达下降,筛选出最佳药物浓度为50μmol /L;黄芩苷干预后miRNA芯片筛选出miR- 126表...

Objective： To observe the effect of Baicalin on the invasion and metastasis of breast cancer and its possible mechanism from the per- spective of miRNA. Methods： The breast cancer cells MCF-7 were treated by 25μmol/L, 50μmol/L, 100μmol/L, 200 μmol/L baicalin. The changes of VEGF and TGF were detected by immunohistochemistry, the best drug concentration was determined; MiRNA chip was used to screen the differentially regulated miRNA, and qRT-PCR was used to verify the up regulation of miRNA. The expressions of Bcl-2, Caspase-9, Caspase-3, ...