期刊论文

Effect of Nrf2 Protein Pathway in Rat＇s Hippocampus with Vascular Dementia and Atherosclerosis Treated by Wu zang Wen yang Hua yu Tang

中文

云南中医学院学报

1000-2723

2013

36

3

8-11+20

10.19288/j.cnki.issn.1000-2723.2013.03.003

国家自然科学基金项目（NO:81160435）；

本校为第一机构

目的 建立动脉粥样硬化血管性痴呆大鼠模型,从氧化应激反应中Nrf2-ARE蛋白通路入手,观察不同剂量五脏温阳化瘀汤对血管性痴呆的神经保护机制.方法 制作动脉粥样硬化血管性痴呆大鼠模型,之后采用反复夹闭大鼠双侧颈总动脉方法制备血管性痴呆大鼠模型.术手后4周将大鼠随机分为假手术组、模型组、中低剂量组、中药中剂量组、中药高剂量组、脑复康组,之后灌胃给药,持续4周,进行Morris水迷宫行为学检测,观察各组大鼠空间学习及记忆能力,western blot检测大鼠海马区Nrf2蛋白细胞核与细胞浆内的表达,同时检测下游蛋白HO-1蛋白的表达.结果 五脏温阳化瘀汤中剂量组和高剂量组从第3天开始可以明显缩短动脉...

Objective To establish a vascular dementia （VaD）model in rats based on atherosclerosis and To observe the treatment of vascular dementia rats with different doses of wu zang wen yang hua yu tang . Explore the mechanism of The decoction of wu zang wen yang hua yu tang from the aspects of oxidative stress response. Methods The rats in experimental groups were injected with a single dose of vitamin D（70U/kg）and loaded with high fat diet,The VaD model was established by repeatedly clipping the rat＇ s common carotid artery. Four weeks later, th...