期刊论文

Wang Yi

中文

生物化学与生物物理进展

1000-3282

2011

38

4

320-330

10.3724/SP.J.1206.2010.00222

The National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30972998]; Hi-Tech Research and Development Program of ChinaNational High Technology Research and Development Program of China [2007AA02Z407]; Hunan Provincial Department of Education [09A079]; Nature Science Found of Hunan Province [07JJY3032]

本校为其他机构

探讨转染人FasL基因的成熟树突状细胞(DC)对异体T淋巴细胞增殖和凋亡的影响,为实现临床器官移植免疫耐受提供初步实验依据.从健康成年人外周静脉血中获得成熟树突状细胞.将人FasL基因成功转染成熟树突状细胞,检测其表面分子的表达和自身凋亡情况,并对其抗原递呈功能进行分析.从异体健康成人外周血中获取T淋巴细胞,将转染成功的树突状细胞与T淋巴细胞混合培养,检测其对T淋巴细胞增殖和凋亡的影响.结果表明:人FasL基因转染没有明显影响成熟树突状细胞表面分子CD40、CD80、CD86和HLA-DR的表达;没有诱导树突状细胞自身发生凋亡;没有影响DC的抗原递呈功能.转染FasL基因后的树突状细胞使异体T淋巴细胞...

To investigate the effects of dendritic cells on T lymphocyte proliferation and apoptosis, providing an in vitro model of clinical transplant immunological tolerance. After mature dendritic cells (mDCs) from peripheral blood of healthy adults was successfully transfected with human FasL gene, mDCs were analyzed for the expression of cell surface molecules, antigen presenting function and their apoptosis. Effects of mDCs on T lymphocyte proliferation and apoptosis were further detected based on co-culture of mDCs and T lymphocytes. The results show that, FasL did not significantly change the ex...