期刊论文

Gu Hong-Feng

中文

生物化学与生物物理进展

1000-3282

2012

39

7

655-661

10.3724/SP.J.1206.2011.00521

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30470720]; Hunan Province Department of Education Fund [09C835]

本校为其他机构

观察鞘内注射姜黄素对坐骨神经慢性压迫性损伤(CCI)大鼠痛阈和脊髓组织Toll样受体4(TLR4)及TNF-α、IL-1β和IL-10表达的影响.鞘内置管的120只大鼠随机均分为4组:假手术组(Sham),CCI组,溶剂对照组(SC),姜黄素治疗组(Cur,100μg/天),建立CCI大鼠疼痛模型,术后第1、3、7、10和14天鞘内给药并测定痛阈,第3、7天取腰段脊髓第4~6节段(L4~L6)以Real-time PCR与Western blotting方法检测TLR4、HMGB1 mRNA和蛋白质的表达,ELISA法观察脊髓组织中TNF-α、IL-1β及IL-10表达变化.与Sham组相比,CCI组大鼠机械性痛阈与热痛阈显著降低(均P

In this study, we investigate the efffects of curcumin trathecal injection on pain threshold and the expressions of TLR4, TNF-alpha, IL-1 beta and IL-10 in the spinal cord of rats with chronic constrictive injury (CCI). 120 males Sprague-Dawley (SD) rats fitted with intrathecal catheters were randomly divided into four groups: sham group (Sham), CCI group (CCI), solvent control group (SC), and curcumin treated group (Cur, 100 mu g/d). Neuropathic pain was produced by CCI of right sciatic nerve as described previously. On the 1st, 3rd, 7th, 10th, and 14th day after surgery, 100 mu g curcumin di...