期刊论文

The Effect and Mechanism of Cytoplasmic M-CSF on Glucose Metabolism in Human Breast Cancer MCF-7 Cells via PI3K/AKT

Tang Sheng-Song;Mo Zhong-Cheng

中文

生物化学与生物物理进展

1000-3282

2019

46

4

398-405

10.16476/j.pibb.2018.0299

Hunan Provincial Science and Technology Plan Key Projects of China [2017SK2183]

本校为其他机构

本文探讨巨噬细胞集落刺激因子(M-CSF)对人乳腺癌MCF-7细胞糖代谢的影响及其机制.构建胞质稳定转染M-CSF的MCF-7细胞(MCF-7-M);ATP检测试剂盒检测MCF-7和MCF-7-M细胞的ATP生成;葡萄糖测定试剂盒、乳酸测试盒检测MCF-7和MCF-7-M细胞的葡萄糖摄取和乳酸分泌情况;蛋白质印迹法检测在糖酵解抑制剂2-脱氧葡萄糖(2-DG)和氧化磷酸化抑制剂OLIG处理后,M-CSF对MCF-7细胞的糖酵解关键酶:己糖激酶2 (HK2)、丙酮酸激酶M2 (PKM2)及葡萄糖转运体1 (GLUT-1)表达的影响;MTT法检测在ATP消耗剂3-溴丙酮酸(3-BrPA)处理后,MCF-7和MCF-7-M细胞对5-FU敏感性的变化.结果发现:MCF-7-M细胞的ATP水平显著高于MCF-7细胞(P

To explore the effect and mechanisms of cytoplasmic M-CSF on glucose metabolism in human breast cancer MCF-7 cells, MCF-7 cells stable expressing cytoplasmic M-CSF were constructed. Relative ATP content was measured by ATP assay kit, glucose was measured using glucose assay kit and lactate was measured using lactate acid assay kit. The expression of HK2, PKM2 and GLUT-1 in three kinds of cell with LY294002 or API-2 was detected by West-blotting. The sensitivity of MCF-7 and MCF-7-M cells to 5-FU with the treatment of ATP depletion by 3-BrPA was observed by MTT assay. It was found that the ATP ...