期刊论文

Celastrol Inhibits Lipid Accumulation by Activating The LXRA/ABCA1 Pathway and Lipophagyin Macrophages

中文

生物化学与生物物理进展

1000-3282

2021

48

7

836-845

10.16476/j.pibb.2020.0388

National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81973668, 81774130, 81670268]; Science Foundation for Distinguished Young Scholars of Hunan ProvinceNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [2018JJ1018]; Science and Technology Project of Changsha City [kq2004060]; Graduate Education Innovation and Professional Ability Improvement Project of Hunan Province [CX20200755]; Hunan The First-Class Discipline of Pharmaceutical Science

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巨噬细胞源性泡沫细胞转化被认为是动脉粥样硬化（atherosclerosis,AS）形成与发展的早期变化,其细胞内蓄积的过量脂质可通过促进血管内膜生长与坏死核形成,继而诱发斑块破裂及血栓形成等严重后果.近期研究发现,雷公藤红素可显著调节脂质代谢,对泡沫细胞转化进程具有潜在的治疗意义.本研究表明,雷公藤红素（Celasrol,CeT）呈浓度依赖性减少巨噬细胞Raw264.7内的脂滴积蓄,并上调胆固醇转运关键分子ABCA1、LXRA蛋白质表达.进一步研究显示,CeT可逆转ABCA1敲低介导的脂质蓄积与ABCA1表达下调.此外,CeT处理Raw264.7细胞24 h时观察到自噬标志物LC3Ⅱ/Ⅰ增加、p62减少,且采用自噬抑制剂3MA可恢复细胞内...

Macrophage-derived foam cell transformation is considered to be an early change in the formation and development of atherosclerosis (AS). Excessive lipid accumulation in the macrophage-derived foam cell often promotes vascular intimal growth and necrosis, which leads to plaque instability and thrombosis. Recent evidence indicated that Celasrol (CeT) can significantly regulate lipid metabolism, which has potential therapeutic significance in the process of foam cell transformation. In this study. CeT treatment can reduce the accumulation of lipid droplets in lipid-loaded Raw264.7 cell in concen...