期刊论文

中文

生物化学与生物物理进展

1000-3282

2022

49

9

1741-1750

10.16476/j.pibb.2021.0211

supported by grants from The National Natural Science Foundation of China (81774362, 81303004) The Natural Science Foundation of Hunan Province (2018JJ2290)

本校为第一机构

目的 探讨左归丸含药血清对化疗损伤性颗粒细胞和膜细胞的影响及作用机制.方法 制备左归丸含药血清,培养大鼠卵巢颗粒细胞和膜细胞,使用磷酰胺氮芥造模分组后给药.CCK-8法测定颗粒细胞和膜细胞存活率,实时荧光定量PCR法(RT-PCR)及蛋白质免疫印迹法(Western blot)分别检测卵巢自噬启动因子Beclin-1、微管结合蛋白轻链3(LC3B)、自噬受体蛋白p62、凋亡蛋白Bax、Caspase3在转录水平和翻译水平上的表达.结果 10％左归丸含药血清对于细胞存活的挽救率最高.Beclin-1、LC3B、Bax、Caspase3在磷酰胺氮芥作用的颗粒细胞和膜细胞中,相对于空白对照组有高表达(P＜0.05),10％左归丸含药血清可下调上述蛋白质在...

Objective To investigate the effect and mechanism of Zuogui pill-containing serum (ZGP-containing serum) on chemotherapy-damaged granular cells (GCs) and theca cells (TCs). Methods GCs and TCs were cultured respectively, the model group was established with phosphoramide mustard (PM), then treated by ZGP-containing serum. The survival rates of GCs and TCs was determined by CCK-8. Real-time fluorescent quantitative PCR (RT-PCR) and Western blot methods were used to detect the expression of Beclin-1, light chain 3 (LC3B), p62, Bax and Caspase3. Results 10% ZGP-containing serum had the best effec...