期刊论文

中文

临床和实验医学杂志

1671-4695

2019

18

2

122-125

10.3969/j.issn.1671-4695.2019.02.003

国家自然科学基金项目(编号:81473615) 湖南省自然基金项目(编号:2015JJ2017).

本校为第一机构

目的 探讨TLR4基因敲除小鼠动脉粥样硬化模型Wnt5a表达的变化及中药护心康的干预作用。方法SPF级TLR4基因敲除小鼠共68只,随机取10只为对照组,予以普通饲料喂养;其余58只予以高脂饲料建立动脉粥样硬化模型,喂养13周后从造模动物中随机选取5只解剖观察其胸主动脉,光镜下发现粥样斑块为造模成功。造模成功动物随机分为模型组、护心康组、阿托伐他汀组,Wnt抑制剂WIF组,每组12只,分别予以相应处理。正常组不做处理,模型组予以生理盐水灌胃并腹腔注射,其余各组均予相应药物灌胃和腹腔注射处理,灌胃容积均为0. 5 ml、腹腔注射容积均为0. 3 ml,每日1次。治疗8周后处死小鼠,心脏采血,酶比色法检测血脂;取...

Objective To investigate Huxinkang intervention in atherosclerosis and changes of Wnt5a.Methods 68 SPF TLR4 gene knockout mice were fed.10 of them were randomly selected as control(normal)group and fed with normal diet.The remaining 58 mice were modeled with high-fat diet for 13 weeks,then,5 mice were observed regarding thoracic aorta.Atheroma plaque was found under light microscopy,indicating successful modeling.The successful modeled mice were randomized into model group,Huxinkang group,atorvastatin group,and Wnt inhibitor WIF group,12 in eac...