期刊论文、会议论文

Myocardial energy metabolism on acute and chronic coronary heart disease in rats with syndrome of blood stasis

中文

中华中医药杂志

1673-1727

2016

31

5

1659-1664

第七届全国中医药博士生学术论坛

第七届全国中医药博士生学术论坛论文集

2016-04-22

合肥

中华中医药学会<&wdkj&>北京岐黄中医药文化发展基金会

国家自然科学基金项目（No.81273670） 教育部博士点基金项目（No.20124323130001） 湖南省研究生创新基金重点项目（No.CX2014A014）~~

本校为第一机构

目的:探讨急、慢性发病过程中冠心病血瘀证心肌能量代谢的变化。方法:分别采用冠脉结扎法和异丙肾上腺素连续注射法结合高脂饲养+维生素D3复制大鼠冠心病急、慢性血瘀证模型,运用GC/TOF-MS对各模型组和对照组大鼠心肌组织进行代谢物检测,利用模式识别进行多元数据处理,筛选差异代谢物,并对差异代谢物进行相关通路分析,筛选差异显著的能量代谢通路。结果:冠心病急、慢性血瘀证各组大鼠心肌组织代谢谱与正常对照组明显不同,差异代谢物涉及能量代谢中重要的糖酵解、脂肪酸代谢和柠檬酸循环,代谢通路分析提示冠心病急、慢性血瘀证过程中能量代谢相关的差异显著通路有淀粉和蔗糖代谢、氨基糖和核苷酸...

Objective: To explore the myocardial energy metabolism on acute and chronic coronary heart disease (CHD) in rats with syndrome of blood stasis based on GC/TOF-MS metabonomics. Methods: The acute and chronic CHD rat model with syndrome of blood stasis were established by high fat diet+vitamin D3 intraperitoneal injection combined with coronary artery ligation and isoproterenol injection repeatedly, respectively. The myocardial metabolites in each group were detected by GC/TOFMS, the multivariate data were analyzed by pattern recognition, and differential metabolites were identified. Consequentl...