期刊论文

Chen, SQ

英文

Microchemical Journal

0026-265X

2025

208

112617

10.1016/j.microc.2024.112617

Natural Science Foundation of Hunan Province [81803964, 82074250, 81973670]; Key Project of the Department of Education of Hunan Province [2023JJ10031, 2020JJ4465, 2020JJ4063]; Xinglin talent cultivation program, Outstanding Innovative Youth Project of Changsha [21A0237]; Western medicine in Hunan Province, Basic medicine construction project of Hunan University of traditional Chinese Medicine [kq2209019, 22JBZ031]; Excellent Teaching Team of Postgraduate in Hunan Province [6]; Hunan Provincial Key Laboratory of Pathogenic Biology of Integrated Traditional Chinese and Western Medicine [[2019] 370-118]; Hunan provincial key laboratory of integrated traditional Chinese and Western medicine [2022KFJJ04]; [2019TP1044]

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A fluorometric bioassay utilizing graphene oxide (GO) is developed with high sensitivity and selectivity for detecting lasR gene transcription (mRNA) in Pseudomonas aeruginosa ( P. aeruginosa ). This approach is formulated by incorporating strand displacement-triggered target recycling and signal amplification facilitated by exonuclease III (Exo III). This system includes a capture probe (CP) labeled with carboxyfluorescein (FAM), an assistant probe (AP), GO, and Exo III, which are involved in generating and amplifying signals. When there is no target, CP is covalently bonded to GO through the...