期刊论文

中文

湖南中医药大学学报

1674-070X

2016

36

11

5-8+17

10.3969/j.issn.1674-070X.2016.11.002

81674025:国家自然科学基金 14JJ3120:湖南省自然科学基金

本校为第一机构

目的观察咳喘宁对RSV诱发哮喘大鼠血清IL-4、IFN-γ的调节作用及对气道平滑肌细胞(airway smooth muscle cells,ASMCs)增殖水平(OD值)的影响。方法将60只大鼠随机分为6组,除正常组外,其余大鼠通过致敏、诱喘、病毒激发哮喘进行造模。造模完成当天分组干预,并用ELISA进行IL-4、IFN-γ水平的测定。造模成功后取支气管平滑肌进行细胞培养,倒置显微镜下化学荧光法鉴定为平滑肌细胞。制备大鼠不同组含药血清并加入第3-7代ASMCs中干预。运用MTT法检测各组ASMCs增殖水平(OD值)。结果 ELISA法结果 ,与模型组相比,西药治疗组、咳喘宁各剂量组血清IL-4、IFN-γ水平差异具有统计学意义(P

Objective To observe the regulating effect of Kechuan Ning on serum IL-4, IFN and the proliferation of airway smooth muscle cells (ASMCs) level (OD level) in RSV induced asthma rats. And to provide experimental basis for the prevention and treatment of asthma. Methods The 60 rats were randomly divided into 6 groups. Except the normal group, the other rats were induced asthma by sensitization and virus to making models. On the day of succesful modeling, the IL-4 and IFN-γ were determined by ELISA. The smooth muscle cells were cultured and the s...