期刊论文

Effect of Zhibai Dihuang Decoction on the expressions of sperm mitochondrial NADH dehydrogenase and ATP synthase in rat model of Ureaplasma urealyticum infection

中文

中国男科学杂志

1008-0848

2016

30

7

3-7

10.3969/j.issn.1008-0848.2016.07.001

81373641:国家自然科学基金 81603634:国家自然科学基金 2015101:湖南省中医药局项目

本校为第一机构

目的研究知柏地黄汤对解脲脲原体(UU)感染模型大鼠精子线粒体NADH脱氢酶mRNA、ATP合成酶mRNA表达的影响。方法取体质量为(220±10)g雄性SD大鼠80只,随机分成假手术组、模型组、中药组(知柏地黄汤)、西药组(强力霉素)、中西药组(知柏地黄汤+强力霉素),造模成功后,假手术组与模型组给予等量的生理盐水灌胃,治疗组(中药组、西药组和中西药组)给予相应的药物灌胃,连续用药21d。末次给药24h后取标本,采用常规精液检测法检测精液质量,RT-PCR法检测精子NADH脱氢酶mRNA、ATP合成酶mRNA表达。结果假手术组、模型组、中药组、西药组、中西药组大鼠精子NADH脱氢酶mRNA表达水平分别为0.44750±0.017078、 0....

Objective To investigate the effects of Zhibai Dihuang Decoction on the expressions of sperm mitochondrial NADH dehydrogenase and ATP synthase in rat model of Ureaplasma urealyticum (Uu) infection. Methods Total of 80 male SD rats with body mass of (220+10)g were randomly divided into sham operation group, model group, Chinese medicine group (Zhibai Dihuang Decoction), western medicine group (doxycycline), western group (Zhibai Dihuang Decoction plus doxycycline), After rat models were successfully established, rats in the sham operation group ...