Objective To study on effect of the total sapongein content of Lilium lancifolium adopting different spreading measure. Methods To determine the sapongein levels in all samples by spectrophotometer with perchloric acid as coloring agents and ultraviolet absorption wavelength at 408 nm. Results The total sapongein levels were between 0.291mg/g -0.836 mg/g in each sample, in which the total sapongeinin was the highest from Lilium browni of Jiangsu province, it was proved to be linear in the range of 50.5 μg - 90.9 μg with the linear equation wa...