期刊论文

中文

现代中药研究与实践

1673-6427

2016

30

6

53-56+67

10.13728/j.1673-6427.2016.06.016

(湘教通[2011]76号):湖南省“十二五”中药学重点学科建设项目资助

本校为第一机构

目的 确定硬脂醇半乳糖苷修饰的水飞蓟宾脂质体的最佳制备工艺,建立高效液相色谱法测定包封率的方法.方法 以包封率为指标,优选乙醇注入法和薄膜分散法制备的硬脂醇苷水飞蓟宾脂质体,并通过单因素考察和正交设计优化处方工艺;用葡聚糖凝胶柱法分离游离药物和脂质体,以HPLC法测定脂质体中水飞蓟宾的包封率.结果 最佳处方为:水飞蓟宾∶氢化大豆磷脂=1∶70,氢化大豆磷脂∶胆固醇=3∶1,水合介质为生理盐水,采用薄膜分散法制成20 ml水飞蓟宾半乳糖化脂质体,包封率为(44.47±0.98)％.结论 薄膜分散法制备硬脂醇半乳糖苷水飞蓟宾脂质体工艺简单可行且包封率较高.

Objective To prepare silybin liposome modified by stearyl galactoside （18-GaI-SLB-LIP）, optimize the prescription technology, and a method of measuring entrapment efficiency was established. Methods Ethanol injection method and film dispersion method were compared by entrapment efficiency as the evaluation index of liposomes. And then single factor study andorthogonal design were adopted to screen the optimal conditions. The encapsulation efficiency of 18-Gal-SLB-LIP was determined by HPLC after the free drug separated by gel chromatography. ...