期刊论文

中文

中国动脉硬化杂志

1007-3949

2016

24

3

239-244

31371161:国家自然科学基金 30971267:国家自然科学基金 12K096:湖南省高等学校创新平台开放基金 14JJ1024:湖南省***基金 2015JJ2117:湖南省***基金

本校为第一机构

目的巨噬细胞凋亡在动脉粥样斑块的发展、病灶的坏死、斑块的不稳定以及急性血管事件中起着重要的作用,因此研究死亡结构相关蛋白（Daxx）对巨噬细胞凋亡的调控作用有利于揭示新的药物靶点,为动脉粥样硬化的发生与发展提供理论依据。方法应用脂质体2000将p CDNA3.1-Daxx、p CDNA3.1转入RAW264.7细胞中,G418（500 mg/L）筛选15天后,建立过表达Daxx稳定细胞株,RT-PCR和Western blot检测稳定细胞株内Daxx的转染效率。模型组和转染的细胞用胆固醇β环糊精（Chol∶MβCD）孵育48 h,建立RAW264.7荷脂和细胞凋亡模型,运用酶荧光法检测细胞内胆固醇蓄积情况,MTT检测细胞活性,流式细胞术观察细胞凋亡。We...

Aim Macrophage apoptosis plays an important role in development of atherosclerotic plaques,necrotic lesions,plaque instability and acute vascular events,so the study of the regulation of Daxx macrophage apoptosis is favorable to reveal new drug targets,providing a theoretical basis for the occurrence of atherosclerosis. Methods The eukaryotic vector of Daxx and p CDNA3. 1 were constructed to be stably transfected into RAW264. 7 cells,the mRNA and protein expressions of Daxx were used by RT- PCR and Western blot respectively. The intracellular l...