期刊论文

中文

中国动脉硬化杂志

1007-3949

2020

28

9

749-756

10.3969/j.issn.1007-3949.2020.09.004

国家自然科学基金项目(81773736)。

本校为第一机构

目的 探讨周期性张应变条件下诱导血管平滑肌细胞(VSMC)表型转换时,心肌素在其中可能的作用.方法 应用FX-5000T体外周期性张应变加载系统,分别对体外培养的VSMC施加频率为1.25 Hz、加载幅度为5％(正常张应变状态)、15％(高张应变状态)的周期性张应变力学刺激,加载时间为24h.采用蛋白免疫印迹法和实时荧光定量PCR技术检测心肌素、肌肉萎缩相关基因1(atrogin-1)及相关收缩蛋白SMA、SM22的蛋白表达水平和mRNA水平;敲除atrogin-1后测心肌素及相关收缩蛋白SMA、SM22的变化;用蛋白酶体抑制剂MG132处理后测心肌素和atrogin-1的表达水平.结果 与5％正常张应变组比较,15％高张应变促进平滑肌细胞的去分化...

Aim To investigate the possible role of myocardin in the vascular remodeling of vascular smooth muscle cell(VSMC)induced by high cyclic tensile stress.Methods Using FX-5000T in vitro cyclic stretch loading system,the VSMC in vitro were stimulated by cyclic stretch with frequency of 1.25 Hz,loading amplitude of 5%(normal physiological state)and 15%(simulated hypertension state)for 24 hours;Western blot and real time RT-PCR were used to detect the protein expression level and mRNA level of myocardin,atrogin-1,SMA and SM22.The expression level of ...