期刊论文

中文

临床肝胆病杂志

1001-5256

2016

32

5

894-898

10.3969/j.issn.1001-5256.2016.05.016

本校为第一机构

目的 观察HBV对体外培养的健康产妇脐带血浆细胞样树突状细胞(pDC)Toll样受体(TLR)9、干扰素调节因子(IRF)7、干扰素(IFN)α表达的影响.方法 体外培养HepG 2.2.15细胞,收集各孔HepG 2.2.15培养上清,超离心HBV病毒颗粒,PCR荧光探针法检测HBV DNA浓度.将培养第7天的pDC与不同载量的HBV(1×105拷贝/ml、1×106拷贝/ml、1×107拷贝/ml和空白对照)共培养24 h.随后每组均加入终浓度为2μg/ml的CpG ODN 2216继续培养24 h.Real Time-PCR检测pDC中TLR9、IRF7 mRNA的表达,Western Blot检测TLR9、IRF7蛋白表达,ELISA法测IFNα水平.计量资料多组间比较采用方差分析,进一步两两比较采用SNK-q检验;对HBV DNA拷...

Objective To investigate the effects of hepatitis B virus（ HBV） on the expression of Toll- like receptor 9（ TLR9）,interferon regulatory factor- 7（ IRF- 7）,and interferonα（ IFNα） in plasmacytoid dendritic cells（ pDCs） cultured in vitro in the umbilical cord blood of healthy parturients. Methods Hep G 2. 2. 15 cells were cultured in vitro,and the culture supernatants of Hep G 2. 2. 15 in each well were collected. HBV particles were obtained by ultracentrifugation,and HBV DNA concentration was measured by fluorescent- probe PCR. pDCs（...