期刊论文

Study on the TCM pathogenesis of nasopharyngeal epithelia carcinogenesis with the use of transgenic techniques

中文

怀化医专学报

2006

1

1-7

国家自然科学基金项目（30271678,30572408）

本校为第一机构

目的 探讨利用转基因技术研究鼻咽上皮细胞癌变中医病机的可行性及相关实践问题。方法 从鼻咽上皮细胞癌变的“气虚染毒”病机假说出发，通过分析其可能的相关性现代生物学基础和代表性指向基因，选取目的基因，显微注射法制备转基因小鼠，反复筛选，建立鼻咽癌前痛变纯系转基因小鼠模型，并以“益气解毒”法进行干预试验。反证模型动物的可靠性。结果 鼻咽癌“气虚染毒”中医病机假说的“气虚”部分与遗传背景有关，“染毒”部分与EB病毒感染活性有关，其可能的相关代表性指向基因分别为体细胞抑癌基因p53和EB病毒瘤基因LMP1。于C57BL／6J♀×CBA♂小鼠转入p53mt和EB病毒LMP1，经反复筛选，获得...

Objective To investigate the TCM pathogenesis of nasopharyngeal epithelia carcinogenesis with the use of transgenic techniques. Methods Based on the hypothesis of Qi deficiency inducing toxin-contamination for clarifying nasopharyngeal epithelia carcinogenesis, target genes responsible for this kind of carcinogenesis were appointed via a careful analysis on the possible modem biological scientific basis underlying thishypothesis and representatively responsible genes associated with it. Microinjection procedures were taken to prepare transgenic mice.Afterwards, repeated screening program was u...