期刊论文

中文

中华老年医学杂志

0254-9026

1996

15

6

358-360

10.3760/cma.j.issn.0254-9026.1996.06.119

本校为第一机构

目的:探讨衰老与生物分子（DNA、胶原蛋白）交联损伤的关系。方法:以Wistar系老年大鼠（18月龄）及青年大鼠（5月龄）为观察对象，取鼠肝提取DNA，测定其熔解温度和增色效应，以反映DNA交联损伤与衰老的关系；取鼠尾腱分别测定尾腱胶原纤维热收缩起始温度、胶原蛋白含量及可溶性胶原含量以反映胶原纤维交联损伤与衰老的关系。结果:老年鼠肝DNA熔解温度（74.3± 1.0℃）和增色效应（1.45±0.08）均高于青年鼠（分别为73.2±1.1℃及1.42±0.03），其中熔解温度的差异有显著性（P值

Objective:To explore the relation between aging and the cross linkage damage of biological molecules (DNA, protein).Method:Old (18 months) and young (5 months) rats of Wistar strain were used and DNA was prepared from the liver of the rats. The melting temperature (Tm) and the hyperchromic effect of DNA were measured. In addition, the thermal contraction temperature, the collagen deposition capacity and the soluble collagen content in caudal tendon were assaied to explore the relation between aging and the cross linkage damage in collagen.Resul...