Objective: In research of tissue autofluorescence spectroscopy, a project focused on analysing autofluorescence properties of large biomolecules using Cr: LiSAF laser as light source was proposed. Methods: xeonpumped Cr: LiSAF tunable second harmonic lasers was adopt to get tunable pulsed blue - light lasers. Laser - in-duced autofiuorescence experiments were constructed consequently. Results: The laser output properties presented show a bright prospect in analysing autofluorescence properties of large biomolecules. Conclusions: Cr: LiSAF laser is suitable in studying the autofluorescence prop...