Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes

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成果类型：

期刊论文

作者：

Yang, Shenghui（杨胜辉）;Brindley, Paul J.;Zeng, Qingren;Li, Yuesheng;Zhou, Jun;...

通讯作者：

Zeng, QR

作者机构：

[Yang, Shenghui; Liu, Yan; Cai, Liting; Li, Yuesheng; Zeng, Tiebing; Lan, Lingmei; Wei, Qi; Zeng, Qingren] Cent South Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha 410013, Hunan, Peoples R China.

[Brindley, Paul J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol &Trop Med, Washington, DC 20037 USA.

[McManus, Donald P.; Li, Yuesheng] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld 4006, Australia.

[Yang, Shenghui] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha 410208, Hunan, Peoples R China.

[Zhou, Jun] Cent South Univ, Xiangya Hosp 3, Expt Ctr, Changsha 410013, Hunan, Peoples R China.

通讯机构：

[Zeng, QR ] C

Cent South Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.

语种：

英文

关键词：

genomic DNA;RNA;telomerase reverse transcriptase;virus glycoprotein;article;cell growth;cell proliferation;gene expression;host parasite interaction;immunohistochemistry;long terminal repeat;nonhuman;parasite;priority journal;reporter gene;Retrovirus;reverse transcription polymerase chain reaction;Schistosoma;Schistosoma japonicum;schistosomiasis;transgene;transgenics;Vesicular stomatitis virus;virion;virus detection;Animals;Animals, Genetically Modified;Cell Line;Genes, Reporter;Humans;Immunoblotting;Immunohistochemistry;Leukemia Virus, Murine;Membrane Glycoproteins;Mice;NIH 3T3 Cells;Polymerase Chain Reaction;Rabbits;Reverse Transcriptase Polymerase Chain Reaction;Schistosoma japonicum;Telomerase;Transduction, Genetic;Transgenes;Viral Envelope Proteins;Murinae;Murine leukemia virus;Oryctolagus cuniculus;Schistosoma;Schistosoma japonicum;Schistosoma mansoni;Vesicular stomatitis virus

期刊：

Molecular and Biochemical Parasitology

ISSN：

0166-6851

年：

2010

卷：

174

期：

页码：

109-116

DOI：

10.1016/j.molbiopara.2010.07.007

基金类别：

We sincerely thank Dr. Dongming Zhou of the Wistar Institute, Philadelphia for the gift of retroviral plasmids, pBABE-puro and pBABE-puro-hTERT, and technical support. We also acknowledge Dr. Robert Weinberg for generously allowing us to use the pBABE-puro-hTERT construct. These studies were supported by Grant No. 30570952 from the Nature Science Foundation of China (NSFC) , and in part by award number R01AI072773 (to PJB) from the NIAID-NIH, USA and the National Health and Medical Research Council of Australia (DPM).

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本校为其他机构

院系归属：

医学院

摘要：

Although draft genome sequences of two of the major human schistosomes, Schistosoma japonicum and Schistosoma mansoni are available, the structures and characteristics of most genes and the influence of exogenous genes on the metabolism of schistosomes remain uncharacterized. Furthermore, which functional genomics approaches will be tractable for schistosomes are not yet apparent. Here, the vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse t...

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Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes

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