Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes

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成果类型：

期刊论文

作者：

Yang, Shenghui（杨胜辉）;Brindley, Paul J.;Zeng, Qingren*;Li, Yuesheng;Zhou, Jun;...

通讯作者：

Zeng, Qingren

作者机构：

[Yang, Shenghui; Liu, Yan; Cai, Liting; Wei, Qi; Zeng, Qingren; Li, Yuesheng; Zeng, Tiebing; Lan, Lingmei] Cent South Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, Changsha 410013, Hunan, Peoples R China.

[Brindley, Paul J.] George Washington Univ, Med Ctr, Dept Microbiol Immunol &Trop Med, Washington, DC 20037 USA.

[McManus, Donald P.; Li, Yuesheng] Queensland Inst Med Res, Mol Parasitol Lab, Brisbane, Qld 4006, Australia.

[Yang, Shenghui] Hunan Univ Chinese Med, Dept Pathogen Biol & Immunol, Changsha 410208, Hunan, Peoples R China.

[Zhou, Jun] Cent South Univ, Xiangya Hosp 3, Expt Ctr, Changsha 410013, Hunan, Peoples R China.

通讯机构：

[Zeng, Qingren] C

Cent South Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.

语种：

英文

关键词：

Schistosoma japonicum;Glycoprotein of vesicular stomatitis virus (VSVG);Pantropic retrovirus;Murine leukemia virus;Transgene;Telomerase

期刊：

Molecular and Biochemical Parasitology

ISSN：

0166-6851

年：

2010

卷：

174

期：

页码：

109-116

DOI：

10.1016/j.molbiopara.2010.07.007

基金类别：

Nature Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [30570952]; NIAID-NIH, USAUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01AI072773]; National Health and Medical Research Council of AustraliaNational Health and Medical Research Council of AustraliaUK Research & Innovation (UKRI)Medical Research Council UK (MRC); NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01AI072773, R01AI072773, R01AI072773, R01AI072773, R01AI072773, R01AI072773, R01AI072773] Funding Source: NIH RePORTER

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

Although draft genome sequences of two of the major human schistosomes, Schistosoma japonicum and Schistosoma mansoni are available, the structures and characteristics of most genes and the influence of exogenous genes on the metabolism of schistosomes remain uncharacterized. Furthermore, which functional genomics approaches will be tractable for schistosomes are not yet apparent. Here, the vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped pantropic retroviral vector pBABE-puro was modified to incorporate the human telomerase reverse transcriptase gene (hTERT) as a reporter, under the...

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Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes

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