期刊论文

Liu, Hao;Jin, J

英文

Microbial Cell Factories

1475-2859

2024

23

1

1-12

10.1186/s12934-024-02306-3

General Project of Hunan Natural Science Foundation [2021JJ30420]; China Hunan Provincial Science & Technology Department [2022NK2005, 2022GK4015, 2022RC1224]; Hunan Provincial Administration of Traditional Chinese Medicine [2021012, A2022002]; Training Program for Excellent Young Innovators of Changsha [kq2206064]; Key project at central government level [2060302]

本校为其他机构

Squalene epoxidase is one of the rate-limiting enzymes in the biosynthetic pathway of membrane sterols and triterpenoids. The enzyme catalyzes the formation of oxidized squalene, which is a common precursor of sterols and triterpenoids. In this study, the squalene epoxidase gene (PcSE) was evaluated in Poria cocos. Molecular docking between PcSE and squalene was performed and the active amino acids were identified. The sgRNA were designed based on the active site residues. The effect on triterpene synthesis in P. cocos was consistent with the results from ultra-high-performance liquid chromato...