期刊论文

Determination of free sugars in Rhizoma Polygonati Odorati by silanization GC/MS

中文

国际药学研究杂志

1674-0440

2018

45

6

472-478

10.13220/j.cnki.jipr.2018.06.012

ZYBZH-Y-HUN-24:国家中医药管理局中医药标准化项目 ZYBZH-Y-HEB-16:国家中医药管理局中医药标准化项目 15K091:湖南省教育厅创新平台开放基金

本校为第一机构

目的 对玉竹的质量标准进行优化.方法 采用气相色谱/质谱联用法(GC/MS)分析来自3个不同产区的玉竹样品中三甲基硅烷(TMS)衍生物组成;采用硅烷化衍生化GC/MS内标法,通过正交实验,以蔗糖和葡萄糖含量为考察指标,对溶剂量、反应温度和反应时间进行筛选,对药材粉末粗细、干燥条件进行优化.结果 最佳硅烷化提取条件参数为0.4 ml〔N,O-双(三甲基硅烷基)三氟乙酰胺(BSTFA):TMCS,99:1〕,反应温度70℃,反应时间20 min.3大主产区玉竹TMS衍生物的共有成分10个,蔗糖、葡萄糖和D-松二糖是组成玉竹游离糖的主要成分;该法的线性相关系数在0.9993～0.9996,加标回收率为90.59％～93.78％,湘玉竹蔗糖含量为0.9687％...

Objective To optimize the quality standard of Rhizoma Polygonati Odorati(RPO). Methods The experiments were performed using RPO samples from three different producing areas. The trimethylsilane(TMS)derivatives of RPOs were separated and determined by GC/MS. The sucrose and glucose were used as reference standards. Using the GC/MS internal standard method with the silylation derivatization,the time,temperature and solvent volume for the silylation reaction and the extraction of silylated de-rivatives were tested by the orthogonal design method. ...