期刊论文

Effects of curcumin on intracellular and extracellular heat shock protein 70 in rat model of thrombosis

中文

国际检验医学杂志

1673-4130

2018

39

24

3006-3008

10.3969/j.issn.1673-4130.2018.24.005

国家自然科学基金资助项目(81473617) 湖南省中医药管理局重点项目(201720).

本校为第一机构

目的 以姜黄提取物为参照研究姜黄素对大鼠血栓模型的影响及其与损伤相关模式分子(DAM P)热休克蛋白70(HSP70)的关系.方法 分别对大鼠进行姜黄素及姜黄提取物灌胃14 d后用物理方法建立大鼠血栓模型,以假手术组、蒸馏水灌胃血栓模型组(血栓模型组)大鼠为对照,获取各组大鼠血液标本和血管组织,采用ELISA法检测各组大鼠血浆HSP70水平,采用免疫组化法检测各组大鼠血管组织中HSP70的表达.结果 血栓模型组大鼠血浆H S P70水平显著高于假手术组大鼠(P=0.003),姜黄素低、高剂量灌胃血栓模型组大鼠血浆HSP70均显著降低(P=0.005、0.002),而姜黄提取物灌胃血栓模型组大鼠血浆HSP70水平无明显变化;与假手术组...

Objective Effects of curcumin on thrombosis model in rats and its relationship with damage-associated model molecule(DAMP)heat shock protein70(HSP70)were studied with turmeric extract as reference.Methods After14days′intragastric administration of curcumin and turmeric extract,thrombosis models were established in rats by physical method.Blood samples and vascular tissues of rats were obtained,and sham operation group and distilled water group as control group.Plasma HSP70levels were detected by ELISA and the expression of HSP70in vascular tis...