期刊论文

Antihepatic-injury effect of crude polysaccharides from roots of Ranunculus ternatus

中文

国际药学研究杂志

1674-0440

2018

45

5

360-366

10.13220/j.cnki.jipr.2018.05.006

国家“十三五”重大新药创制专项课题（2017ZX10205504）；

本校为第一机构

目的研究猫爪草多糖对肝损伤的保护作用。方法猫爪草药材依次经60℃和100℃水提获得水提液,2种水提液分别经乙醇沉淀、透析和冷冻干燥方法制备2种粗多糖(命名为RTP-60和RTP-100);采用刀豆蛋白A(ConA)诱导建立小鼠免疫性肝损伤模型、人LO2肝细胞和HepG2.2.15肝细胞损伤模型,评价粗多糖的抗肝损伤活性。结果与ConA诱导的免疫性肝损伤模型小鼠相比,灌胃给予RTP-60或RTP-100(500 mg/kg)均能明显减轻小鼠肝组织病理性损伤,降低小鼠肝中天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性(P

Objection To investigate the hepatoprotective effect of crude polysaccharides from the root of Ranunculus ternatus. Methods The roots of R. ternatus were extracted with water in turn at 60℃ and 100℃ to obtain two water-extracted solutions at 60℃ and 100℃,both of which were concentrated into 1 L,respectively. Then,two crude polysaccharides,RTP-60 and RTP-100, were obtained from the water-extracted solutions at 60℃ and 100℃,respectively,through precipitation with ethanol,dialysis and freeze-drying processes. The hepatoprotective effects of ...