期刊论文

Study on Huangqi Jiedu Decoction for Wnt/β-catanin signaling pathway inhibiting EMT against lung metastasis of breast cancer

中文

国际中医中药杂志

1673-4246

2023

45

9

1113-1118

10.3760/cma.j.cn115398-20221208-00125

本校为第一机构

目的:观察黄芪解毒汤对荷瘤裸鼠乳腺癌肺转移的抑制作用，探讨Wnt/β-连环蛋白（β-catenin）通路调控上皮间质转化（EMT）的作用机制。方法:将30只裸鼠按随机数字表法分为模型组、阿霉素组及黄芪解毒汤低、中、高剂量组，每组6只。各组通过皮下注射小鼠乳腺癌4T1细胞制备荷瘤裸鼠模型。建模成功后，黄芪解毒汤低、中、高剂量组灌胃黄芪解毒汤17.82、35.64、71.28 g/kg，阿霉素组腹腔注射阿霉素0.05 g/kg，模型组灌胃等体积的生理盐水，均连续给药21 d。于造模后9、15、21 d测定瘤体积；给药结束后分离肿瘤组织，称瘤重、计算抑瘤率；分离肺组织，计数肺转移瘤结节数，计算肺转移抑制率；HE染色...

Objective:To observe the inhibitory effects of Huangqi Jiedu Decoction on lung metastasis of breast cancer in nude mice; To explore the mechanism of intervening epithelial mesenchymal transformation (EMT) induced by Wnt/β-catenin signaling pathway.Methods:Totally 30 nude mice were divided into model group, adriamycin group and Huangqi Jiedu Decoction low-, medium-, and high-dosage groups according to random number table method. Each group was injected subcutaneously with mouse breast cancer 4T1 cells to construct tumor - bearing nude mice mode...