期刊论文

中文

湖南中医药大学学报

1674-070X

2009

29

6

22-25

10.3969/j.issn.1674-070X.2009.06.007

湖南省科技厅资助项目（2007TP4021和湘财教指2009-42）； 湖南省教育厅资助项目（08C637;09C724）；

本校为第一机构

目的 研究壮骨镇痛胶囊对高转移人乳腺癌MDA-MB-231细胞体外增殖和迁移的影响.方法 采用血浆药理学方法制备壮骨镇痛胶囊含药血浆,采用MTT法检测不同浓度含药血浆对MDA-MB-231细胞生长增殖的影响;采用划痕法测定其对MDA-MB-231细胞迁移的影响.结果 当正常大鼠血浆浓度≤15.0%时,对MDA-MB-231细胞生长增殖无明显影响,差异无统计学意义(P＞0.05);壮骨镇痛胶囊含药血浆浓度在5.0%～15.0%范围内,对MDA-MB-231细胞生长增殖均有显著抑制作用,差异有统计学意义(P＜0.01或P＜0.05),该药对MDA-MB-231细胞的最高非毒性浓度为2.5%:当壮骨镇痛胶囊含药血浆浓度为5.0%和1.25%时能显著抑制乳腺癌细胞的迁移,差...

Objective To study the inhibitory effects of Zhuanggu Zhentong capsules（ZZC） on the growth and migration of MDA-MB-231 in breast cancer cell lines in vitro. Methods The drug-containing ZZC plasma was prepared with serologic pharmacological method. The effects of different doses of ZZC on the growth of MDA-MB-231 ceils was detected with MTT method and on the migration of MDA-MB-231 cell measured with scarification method. Results There were no obvious effects on the growth of MDA-MB-231 cells when the drug-containing plasma concentration was ≤...