期刊论文

中文

中医药导报

1672-951X

2014

20

8

18-21

10.13862/j.cnki.cn43-1446/r.2014.08.007

No.81173264:国家自然科学基金 No.20110491256:中国博士后科学基金 No.11A088:湖南省教育厅重点项目 No.10A089:湖南省教育厅重点项目

本校为第一机构

目的:探讨不同浓度麝香配伍乳香含药血浆（DP-M&O）对单核细胞分泌TNF-α、IL-1β、IL-6、IL-8、IL-10水平的影响,了解麝香配伍乳香在治疗前列腺炎中抗炎作用的机理。方法:制备小鼠脾脏单核细胞,细胞培养后分加入2.5%、5%、10%、20%浓度的DP-M&O预处理1 h后,加入小鼠前列腺抗原（PAgs）诱导活化单核细胞。阳性对照组加入脂多糖（LPS）处理。阴性对照组加入等体积DMEM完全培养基。培养96 h后收集各组上清,ELISA法检测单核细胞分泌的炎症因子TNF-α、IL-1β、IL-6、IL-8、IL-10的水平。结果:与空白对照组比较,经PAgs刺激诱导后,各组小鼠单核细胞的炎症因子释放增加,除IL-10的升高明显低于阳性对照...

Objective： To study the compatibility of musk and olibanum, medicated serum（DP-MO） of different concentration on TNF-α, IL-1β, IL-6, IL-8, and IL-10 secreted by monocyto induced by PAgs, in order to understand the mechanism of musk and olibanum of anti-inflammatory in the treatment of prostatitis. Methods： The mice spleen monocytes was cultured after preparation, and 2.5%, 5%,10% and 20%concentration of DP-MO were respectively added for pretreatment, with addition of mouse prostate specific antigen（PAgs） in 1 h. LPS was added into posit...